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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has actually been observed in many tumors, which ends up in chromosome instability, and so could contribute to tumorigenesis. This protein was identified to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a crucial part in marketing mobile-cycle development during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, are actually explained. Two extra splice variants were being claimed but specific nucleotide sequence data will not be but accessible.
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Blocking peptides are peptides that bind especially into the target antibody and block antibody binding. These peptide usually incorporates the epitope acknowledged via the antibody.
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This guide outlines even more possible optimization of cellular and immunoassay parameters to be certain the best possible success are...
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This tutorial outlines further feasible optimization of mobile and immunoassay parameters to be certain the very best success are...
Antibodies sure to the blocking peptide no longer bind for the epitope within the concentrate on protein. By comparing the staining from the blocked antibody versus the antibody by itself, one can see which staining is certain.
Focus on Facts Cyclin E belongs to your extremely conserved cyclin loved ones, whose members are characterised by a spectacular periodicity in protein abundance in the cell cycle. Cyclins functionality as regulators of CDK kinases. Distinct cyclins show distinct expression and degradation styles which contribute to your temporal coordination of each and every mitotic event. Cyclin E varieties click here a complex with and capabilities like a regulatory subunit of CDK2, whose activity is needed for cell cycle G1/S transition. This protein accumulates at the G1-S stage boundary and is degraded as cells development through S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...
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